Our standard pump is Cole Parmer 7528-10, 0 to 600 rpm
Our standard head is 77200-62. High performance (thick wall) tubing only: 15, 24, 35, 36
The pumps used to be controlled by software, but that was far too complex and prone to failure. Since we have moved to lower-pressure perfusion, controlling the pump speeds is much simpler. We sometimes manually control the pump speeds using the buttons on the pumps. Other times, we use a simple 1kohm potentiometer with a 10mA (100mW) draw connected to a 10V power supply.
We use four pins from the DB9 connector. Pin 1 is labeled on the back of the pump as the upper right corner of the female connector. Pin numbers are also on all connectors.
|1||red||Speed Control Voltage Input 0 to +10V|
|3||white||Speed Control Input ground (-) reference (not an earth ground)|
|6||green||CW/CCW ground (-) reference (seems to be an earth ground) (no longer used)|
|8||black||CW/CCW +5V (clockwise/counterclockwise) (no longer used)|
In addition, the following two pins must be jumpered together:
6/7: This is required to tell the pump to run when in EXT mode, as it will be when we are controlling the speed via the 0-10V input. The front start/stop control will not work. Instead, the user must hit the Int/Ext button in order to manually stop the pump via the panel. Five different ways of stopping the pump are listed on the Software Perfusion Monitor page.
This is one way to wire it, although each situation is different:
What's not shown above is how the two green wires are then joined, soldered and insulated with shrink tubing a few inches from the connector. Alternately, pins 6 and 7 could be just be bridged with a short piece of wire if the quality is good. The shrink tubing shown in the picture is not always necessary. It's generally used inside the Wiring Box, even covering the solder connections more than shown. It's generally not used inside cables as long as the wires are not too flexible. It's preferrable to use store bought M/F DB9 extension cables. Backup cables of different lengths are kept on hand.
The pump used by ANB must have pins 1 - 3 bridged. Pin 5 is the hot wire.
ISMATEC Reglo ICC is a double-headed pump used by ANB. It does not support voltage input, so it is hooked up to a USB to Serial Converter and is run with ASCII commands. After plugging in, go to PC, right click, Manage, Device Manager, Ports. Find the USB to Serial Converter, and make a note of the COM#. Use that in the Config File. Support is limited to a single double-headed pump that is not mixed with other pumps.
Reglo flow was calculated as follows. In the Reglo ICC Operating Manual, on page 16, it lists tubing order number SC0072T. This is the only tubing that is currently supported. This tubing has an ID of 2.79, and a flow rate of 31 mL/min at 100 rpm.
The old manual pump is 7554-80, economy 7 to 200 rpm
Old head is 7518-10, easy-load, compatible with L/S 25, L/S 17, L/S 18, and others.